Part:BBa_K5308006

HapAmp backbone for integration in yeast genome with high copynumber

A method that uses haploinsufficiency as evolutionary force to drive in vivo gene amplification.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2650
  Illegal XbaI site found at 1874
  Illegal SpeI site found at 5602
  Illegal PstI site found at 1140
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2650
  Illegal NheI site found at 817
  Illegal SpeI site found at 5602
  Illegal PstI site found at 1140
  Illegal NotI site found at 2062
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2650
  Illegal BglII site found at 1709
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2650
  Illegal XbaI site found at 1874
  Illegal SpeI site found at 5602
  Illegal PstI site found at 1140
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2650
  Illegal XbaI site found at 1874
  Illegal SpeI site found at 5602
  Illegal PstI site found at 1140
  Illegal AgeI site found at 1045
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 1742
  Illegal BsaI site found at 3966
  Illegal BsaI.rc site found at 1497
  Illegal SapI.rc site found at 5048